猴痘病毒
猴痘病毒(Mpox virus，MPXV)與天花病毒一樣，屬于正痘病毒屬。其形態(tài)與正痘病毒一致, 外形為圓角磚形或卵圓形,大小為200~400nm。磚形或卵圓狀，外被脂蛋白膜或封套,中間具有兩個含蛋白質的側體,有1個厚膜核心,核心內含很大的雙鏈DNA基因體。M痘病毒的基因組為雙鏈DNA,長約197kb,基因組末端包含一個相同但方向相反的末端反向重復序列。該病毒包含190個開放閱讀框,其中4個位于末端反向重復序列中。猴痘病毒核心蛋白為A29L, A35R, B6R, M1R, H3L
法國ProteoGenix頂級科學團隊專注于抗體藥物開發(fā)，旗下科研試劑品牌AntibodySystem專注于蛋白和抗體試劑研究，團隊對猴痘病毒(Mpox virus，Mpox)和痘苗病毒(vaccinia virus,VACV)的基因序列進行深度探索并成功設計開發(fā)真核系統(tǒng)表達的猴痘病毒(Mpox virus，Mpox)系列核心蛋白A29L、A35R、B6R、M1R、E8L以及相關具有生物學活性的中和抗體產品，助力疫苗、藥物、診斷試劑等不同方向的研究。
真核表達猴痘核心蛋白及相關中和抗體Monkeypox virus/MPXV A29L
A29L是細胞內成熟病毒（IMV）表面包膜蛋白（與牛痘病毒VACV,A27同源）。溶瘤痘苗病毒(VV)屬于痘病毒科中一擁有192kb雙螺旋DNA遺傳物質的病毒,會在細胞質中繁殖出具有鞘膜的病毒,進而感染其他不同種的哺乳動物細胞及個體.VV中的A27L基因能表達出一14kDa大小的鞘膜蛋白,此蛋白能與細胞表面上的硫酸乙酰肝素（Heparan Sulfate）作用使病毒跟細胞結合并且可催化細胞融合。
·Monkeypox virus/MPXV A29L|
Catalog No：EVV13401 |
Name:Recombinant Monkeypox virus/MPXV A29L Protein, C-His |
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Predicted molecular weight：15.33 kDa |
Expression system: Mammalian |
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Protein length: Asp2-Glu110 |
Protein length: Asp2-Glu110 |
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SDS PAGE Purity：90% SDS PAGE for Recombinant Monkeypox virus/MPXV A29L Protein |
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Catalog No：RVV13401 |
Name：Anti-Monkeypox virus/MPXV A29L Antibody (SAA0286） |
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Host species：Mouse |
Applications：ELISA,Neut |
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Species reactivity:MPXV |
Stock：In stock |
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Monkeypox virus/MPXV A35R
A35R是細胞內包膜病毒粒子（IEV）和細胞外包膜病毒粒子（EEV）的包膜成分，與脊索痘病毒A33R同源。痘苗病毒中的A33R在病毒粒子在細胞間的傳播中起著至關重要的作用，是疫苗開發(fā)的重要靶點，也有研究表明A35R是猴痘病毒(Monkeypox virus，MPXV)血清學檢測的潛在靶點。
·Recombinant Monkeypox virus/MPXV A35R Protein|
Catalog No：EVV13101 |
Name:Recombinant Monkeypox virus/MPXV A35R Protein, C-His |
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Predicted molecular weight：16.65 kDa |
Expression system: Mammalian |
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Protein length: Val57-Thr181 |
Stock：In stock |
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SDS PAGE Purity：90% SDS PAGE for Recombinant Monkeypox virus/MPXV A35R Protein |
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Catalog No：RVV13101 |
Name:Anti-Monkeypox virus/MPXV A35R Antibody (SAA0287) |
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Host species：Mouse |
Applications：ELISA,Neut |
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Species reactivity:MPXV |
Stock：In stock |
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Detects Monkeypox virus/MPXV A35R in direct ELISAs. |
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Monkeypox virus/MPXV B6R
B6R是宿主范圍蛋白，位于細胞外包膜病毒粒子（EEV）的膜上，與牛痘病毒補體控制蛋白B5同源，參與補體激活的負調節(jié)。研究表明B5依賴于與A33或A34的相互作用，以定位到細胞內包膜的位點，并能相互結合釋放到細胞外病毒體的包膜中。B5對于有效生產感染性EV至關重要，A33，A34，B5三者復合物在ER退出以及三種糖蛋白正確定位到包裹的細胞內部位中起作用。
·Recombinant Monkeypox virus/MPXV B6R/SL-159 Protein|
Catalog No：EVV13501 |
Name:Recombinant Monkeypox virus/MPXV B6R/SL-159 Protein, C-His |
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Predicted molecular weight：31.73 kDa |
Expression system: Mammalian |
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Protein length: Thr20-His279 |
Stock：In stock |
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SDS PAGE Purity：90% SDS PAGE for Recombinant Monkeypox virus/MPXV B6R/SL-159 Protein |
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Monkeypox virus/MPXV M1R
M1R與牛痘病毒L1蛋白同源，是一種在成熟IMV顆粒表面發(fā)現(xiàn)的跨膜蛋白。由L1R ORF編碼，高度保守，參與病毒粒子組裝，在病毒進入和成熟過程中發(fā)揮重要作用。L1是誘導低pH觸發(fā)的細胞-細胞融合所必需的，且對牛痘病毒復制至關重要。
·Recombinant Monkeypox virus/MPXV M1R Protein|
Catalog No：EVV13301 |
Name:Recombinant Monkeypox virus/MPXV M1R Protein, C-His |
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Predicted molecular weight：22.35 kDa |
Expression system: Mammalian |
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Protein length: Gly2-Gly183 |
Stock：In stock |
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SDS PAGE Purity：90% SDS PAGE for Recombinant Monkeypox virus/MPXV M1R Protein |
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Catalog No：RVV13301 |
Name:Anti-Monkeypox virus/MPXV M1R Antibody (SAA0283) |
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Host species：Mouse |
Applications：ELISA,Neut |
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Species reactivity:MPXV |
Stock：In stock |
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Detects Monkeypox virus/MPXV M1R in direct ELISAs. |
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Monkeypox virus/MPXV E8L
E8L是病毒入侵宿主細胞重要基因，在病毒入侵宿主完成復制的過程中作為MV的細胞表面結合蛋白與細胞表面的硫酸軟骨素結合，以提供病毒粒子附著在靶細胞上。有研究表明利用siRNA技術能有效拮抗Monkeypox virus的A6R和E8L基因，由此構建siA6-a和si8 -d能破壞MPV復制。
·Recombinant Monkeypox virus/MPXV E8L Protein|
Catalog No：EVV13201 |
Name:Recombinant Monkeypox virus/MPXV E8L Protein, C-His |
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Predicted molecular weight：22.35 kDa |
Expression system: Mammalian |
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Protein length: Gly2-Gly183 |
Stock：In stock |
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SDS PAGE Purity：90% SDS PAGE for Recombinant Monkeypox virus/MPXV E8L Protein |
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Catalog No：RVV13201 |
Name:Anti-Monkeypox virus/MPXV E8L Antibody (SAA0285) |
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Host species：Mouse |
Applications：ELISA,Neut |
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Species reactivity:MPXV |
Stock：In stock |
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Detects Monkeypox virus/MPXV E8L in direct ELISAs. |
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Monkeypox virus/MPXV H3L
H3L是一種324個AA長的糖基轉移酶，位于病毒包膜上，在與宿主細胞的附著和隨后的痘病毒進入中起關鍵作用。研究表明，天花疫苗誘導的抗體與H3蛋白結合，分化簇 (CD8)+ T細胞將H3L識別為抗原，表明其免疫學意義。
·Recombinant Monkeypox virus/MPXV H3L Protein|
Catalog No：EVV12701 |
Name:Recombinant Monkeypox virus/MPXV H3L Protein, C-His |
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Predicted molecular weight：35.46 kDa |
Expression system: Mammalian |
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Protein length: Ala2-Phe282 |
Stock：In stock |
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SDS PAGE Purity：90% SDS PAGE for Recombinant Monkeypox virus/MPXV H3L Protein |
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隨著研究的深入，AntibodySystem還研發(fā)了一系列對猴痘病毒（Monkeypox virus，MPXV ）相關檢測試劑盒產品。
猴痘病毒（Monkeypox virus/MPXV ）抗原檢測試劑盒
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Cat. # |
Product name |
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KVV13401 |
Monkeypox Virus (MPXV) A29L Antigen ELISA Kit |
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KVV13301 |
Monkeypox Virus (MPXV) M1R Antigen ELISA Kit |
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KVV12701 |
Monkeypox Virus (MPXV) H3L Antigen ELISA Kit |
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KVV13201 |
Monkeypox Virus (MPXV) E8L Antigen ELISA Kit |
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KVV13101 |
Monkeypox Virus (MPXV) A35R Antigen ELISA Kit |
猴痘病毒（Monkeypox virus/MPXV ）抗體檢測試劑盒
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Cat. # |
Product name |
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KAV13401 |
Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit (A29L) |
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KAV13301 |
Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit(M1R) |
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KAV12701 |
Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit(H3L) |
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KAV13201 |
Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit(E8L) |
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KAV13101 |
Human Anti-Monkeypox Virus (MPXV) IgG ELISA Kit(A35R) |
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參考文獻：
[1]Subunit recombinant vaccine protects against monkeypox. J Immunol. 2006 Aug 15;177(4):2552-64. doi: 10.4049/jimmunol.177.4.2552. PMID: 16888017.
[2]Outbreak of human monkeypox in Nigeria in 2017-18: a clinical and epidemiological report. Lancet Infect Dis. 2019 Aug;19(8):872-879. doi: 10.1016/S1473-3099(19)30294-4. Epub 2019 Jul 5. PMID: 31285143.
[3]A Nucleic Acid-Based Orthopoxvirus Vaccine Targeting the Vaccinia Virus L1, A27, B5, and A33 Proteins Protects Rabbits against Lethal Rabbitpox Virus Aerosol Challenge. J Virol. 2022 Feb 9;96(3):e0150421. doi: 10.1128/JVI.01504-21. Epub 2021 Dec 1. PMID: 34851148; PMCID: PMC8826804.
[4]Mapping and functional analysis of interaction sites within the cytoplasmic domains of the vaccinia virus A33R and A36R envelope proteins. J Virol. 2003 Apr;77(7):4113-26. doi: 10.1128/jvi.77.7.4113-4126.2003. PMID: 12634370; PMCID: PMC150634.
[5]Vaccinia virus induces cell fusion at acid pH and this activity is mediated by the N-terminus of the 14-kDa virus envelope protein. Virology. 1990 Sep;178(1):81-91. doi: 10.1016/0042-6822(90)90381-z. PMID: 2389560.
[6]Molecular Evidence of Human Monkeypox Virus Infection, Sierra Leone. Emerg Infect Dis. 2019 Jun;25(6):1220-1222. doi: 10.3201/eid2506.180296. Epub 2019 Jun 17. PMID: 30900976; PMCID: PMC6537715.
[7]Inhibition of monkeypox virus replication by RNA interference. Virol J. 2009 Nov 4;6:188. doi: 10.1186/1743-422X-6-188. PMID: 19889227; PMCID: PMC2777875.
[8]Rapid protection in a monkeypox model by a single injection of a replication-deficient vaccinia virus. Proc Natl Acad Sci U S A. 2008 Aug 5;105(31):10889-94. doi: 10.1073/pnas.0804985105. Epub 2008 Aug 4. PMID: 18678911; PMCID: PMC2495015.
