Description
IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). It cleaves Immunoglobulin G (IgG) with high specificity at a single site below the hinge region, yielding F(ab’)2 and Fc fragments.

Catalog No.
YXX05001

Form
Liquid

Storage buffer
0.01M PBS, pH 7.4, 50% glycerol, 0.05% Proclin 300.

Concentration
20000U/mg

Purity
90% as determined by SDS-PAGE

Applications
Proteomics

Endotoxin level
Please contact with the lab for this information.

Expression system
E. coli

Protein length
IdeS Protease is cloned from Streptococcus pyogenes and expressed in E.coli.

Nature
Recombinant

Predicted molecular weight
37.57 kDa

Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.

Precision
Unit Definition

One unit will cleave ≥95% of 1μg of recombinant monoclonal IgG in 30 minutes at 37°C.

 

Digestion reaction condition

37℃, 30min

 

Protocol

1. Add appropriate amount of IgG (to 5mg) in digestive juice;

2. Add IdeS protease to IgG samples: add 1 unit of IdeS per 1ug of IgG;

3. Incubate the sample at 37°C for 30-60min.

* IdeS proteases are most active in buffers at or near neutral pH. The recommended reaction buffer is 50 mM sodium phosphate and 150 mM NaCl (pH 6.6), but most common biological buffers are suitable, such as Tris or PBS. Buffers outside this pH range (such as acetate buffer) may also be suitable, but the incubation time or enzyme amount needs to be optimized according to the actual situation.

Species
Streptococcus pyogenes

Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Note
For research use only.