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| 產地 | France |
| 保存條件 | Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at -20 to -80 °C for twelve months from the date of receipt. |
| 品牌 | AntibodySystem |
| 貨號 | CLH00034 |
| 用途 | For research only |
| 組織來源 | 咨詢 |
| 細胞形態(tài) | 咨詢 |
| 是否是腫瘤細胞 | 否 |
| 保質期 | 1 year |
| 器官來源 | 咨詢 |
| 免疫類型 | 咨詢 |
| 品系 | 咨詢 |
| 生長狀態(tài) | 咨詢 |
| 物種來源 | 咨詢 |
| 包裝規(guī)格 | 100 μg at 2 mg/ml |
| 是否進口 | 否 |
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HCT116 whole cell lysate(細胞裂解液)
CLH00034
Liquid
1 X Sample Buffer (1X RIPA buffer + 1X SDS loading buffer).
100 μg at 2 mg/ml
WB
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at -20 to -80 °C for twelve months from the date of receipt.
Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer (50mM Tris(pH 7.4)，150mM NaCl，1% Triton X-100, 1% deoxycholate, 0.1% SDS) with cocktail of protease inhibitors. Cell debris was removed by centrifugation. Protein concentration was determined by Bradford assay. The cell lysate was boiled for 5 min in 1 x SDS loading buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol.
Aiming to develop the most reliable bioreagents, AntibodySystem SAS was founded in 2019 in France by a team of professionals from top industrial companies and research institutes. The collective scientific and technical experiences in our team have brought breaking throughs in multiple product developments, including recombinant antibodies, recombinant proteins, biosimilar, RUO ELISA kits and other related products. By working with talented researchers and technicians, AntibodySystem continues to upgrade product qualities and lower the overall cost, offering accountable and affordable solutions for biolabs all over the world. Since its establishment, AntibodySystem has built long term and stable collaborations with academia, pharmaceutical industry, biotech companies and research hospitals, supporting their R&D by AntibodySystem's bioreagents with excellent data accuracy and reproducibility.
AntibodySystem由具有30多年蛋白抗體開發(fā)經驗的 創(chuàng)立于法國,專注于生命科學和生物制藥領域研究,總部位于法國斯特拉斯堡市。AntibodySystem自主開發(fā)了高效、高產的真核重組表達系統(tǒng),利用該系統(tǒng)生產了高質量的重組蛋白、抗體產品。目前產品包括藥物靶點蛋白以及對照抗體藥物、Invivo功能性抗體、SAA系列流式抗體、PEG抗體、磷酸化抗體、抗小分子抗體、ADA抗體、PK&ADA ELISA試劑盒。
普健生物作為AntibodySystem在亞洲區(qū)授權 ,致力于為廣大科研工作者提供 的產品服務。
如果您對我們的產品感興趣,請聯系027-65279366。
以上產品僅用于科研
